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Part:BBa_K4877016

Designed by: Team iGEM Bielefeld-CeBiTec 2023   Group: iGEM23_Bielefeld-CeBiTec   (2023-10-12)


DART VADAR sensor cloning site

This part is the sensor cloning site of the published DART VADAR circuit by Gayet et al. [1]. It can be used to clone a sensor insert into the DART VADAR open reading frame by HindIII digestion followed by Gibson assembly.

The only change introduced is a mutation of an illegal PstI restriction site in the left homology arm.

[1] Gayet, R.V., Ilia, K., Razavi, S. et al. Autocatalytic base editing for RNA-responsive translational control. Nat Commun 14, 1339 (2023). https://doi.org/10.1038/s41467-023-36851-z

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 52
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1
    Illegal XhoI site found at 44
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None